The slides used in the Tuesday and Thursday lectures will be posted here, shortly after the class.

Lecture 1, 9 January

Lecture 2, 11 January

Lecture 3, 16 January

Lecture 4, 18 January

Lecture 5, 23 January

  • The Beer-Lambert Law and Measuring Protein Concentrations
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Lecture 6, 25 January

Lecture 7, 30 January

Lecture 8, 1 February

Lecture 9, 6 February

  • X-ray Crystallography and the Catalytic Triad of the Serine Proteases
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Lecture 10, 8 February

Lecture 11, 13 February

Lecture 12, 15 February

Lecture 13, 20 February

  • Fitting the Michaelis-Menten Equation and Interpretation of Km and kcat.
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Lecture 14, 22 February

  • Energy Profiles for Enzyamatic Reactions and Introduction to Enzyme Inhibitors
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Lecture 15, 27 February

Lecture 16, 1 March

  • β-secretase Inhibitors and Irreverisble Serine-Protease Inhibitors
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Lecture 17, 6 March

  • Acetylcholine Esterase Inhibitors and Irreversible Enzyme Inhibition Kinetics
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Lecture 18, 8 March

  • Analysis of Reversible Inhibitor Data and an Exosite Inhibitor
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Lecture 19, 13 March

  • Introduction to Separation Methods and Thiol-disulfide Chemistry
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Lecture 20, 15 March

  • More on Thiol-Disulfide Chemistry: The Anfinsen Experiment
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Lecture 21, 27 March

Lecture 22, 29 March 2018

Lecture 23, 3 April

Lecture 24, 5 April

  • Analysis of SDS Gels and Some Other Electrophoretic Techniques
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Lecture 25, 10 April

  • Introduction to Chromatography and Gel Filtration
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  • Chromatography Handbooks Many of the chromatography media most commonly used for biochemistry were developed by Pharmacia Fine Chemicals, now a part of GE Healthcare. For many years, Pharmacia published free handbooks on the use of these materials, and updated versions are available for download. The handbooks for size exclusion (gel filtration) and ion exchange chromatography are particularly useful:

Lecture 26, 12 April

  • More on Gel Filtration Chromatography, and the Trypsin Resurrection Experiment
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Lecture 27, 17 April

  • Trypsin Substrates, Chromatography and the Ultracentrifuge
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Lecture 28, 19 April